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微波诱变结合抗性突变筛选放线菌素D高产菌株
引用本文:任林英,张祝兰,王徳森,杨煌建,唐文力,孙菲. 微波诱变结合抗性突变筛选放线菌素D高产菌株[J]. 生物技术进展, 2015, 5(5): 377-381. DOI: 10.3969/j.issn.2095-2341.2015.05.09
作者姓名:任林英  张祝兰  王徳森  杨煌建  唐文力  孙菲
作者单位:福建省微生物研究所, 福建省新药(微生物)筛选重点实验室, 福州 350007
基金项目:福建省科技项目(2014R1009-10) 资助。
摘    要:采用微波结合链霉素抗性筛选法选育放线菌素D的高产菌株。通过考察链霉素对Streptomyces rubiginosohelvolus FIM-N31菌株孢子生长情况的影响确定链霉素致死浓度,出发菌株FIM-N31的孢子经微波辐照处理后,涂布在含链霉素致死浓度(50 μg/mL)的培养基平板上培养,获得了大量的链霉素抗性基因突变株。摇瓶发酵筛选突变株,结果获得一株遗传性状稳定的放线菌素D高产菌Str186,其产放线菌素D的能力比出发菌株提高了8倍以上。

关 键 词:链霉菌  放线菌素 D  抗生素抗性筛选  复合诱变  
收稿时间:2015-05-08

Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis
REN Lin-ying,ZHANG Zhu-lan,WANG De-sen,YANG Huang-jian,TANG Wen-li,SUN Fei. Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis[J]. CURRENT BIOTECHNOLOGY, 2015, 5(5): 377-381. DOI: 10.3969/j.issn.2095-2341.2015.05.09
Authors:REN Lin-ying  ZHANG Zhu-lan  WANG De-sen  YANG Huang-jian  TANG Wen-li  SUN Fei
Affiliation:Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China
Abstract:Microwave mutagenesis and streptomycin resistance screening was applied to screen actinomycin D high-producting strain. After testing the resistance of Streptomyce rubiginosohelvolus FIM-N31 to streptomycin, the lethal concentration of streptomycin was determined. A lot of streptomycin-resistant mutants were screened after the spores regenerated on the lethal streptomycin concentration (50 μg/mL) media when they were treated with microwave irradiation. It was eventually found that the high-yield strain Str186 from these mutants which was stably inheritable, and the productivity was beyond eight times higher than the original strains in the rotation-flask experiments.
Keywords:Streptomyces  actinomycin D  antibiotics resistance gene screening  compound mutation  
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