Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions |
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| Authors: | Anthony R Poteete |
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| Institution: | (1) Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA |
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| Abstract: | Background Previous studies of gene amplification in Escherichia coli have suggested that it occurs in two steps: duplication and expansion. Expansion is thought to result from homologous recombination
between the repeated segments created by duplication. To explore the mechanism of expansion, a 7 kbp duplication in the chromosome
containing a leaky mutant version of the lac operon was constructed, and its expansion into an amplified array was studied. |
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