Atlantic salmon (Salmo salar L.) skin contains a novel kininogen and another cysteine proteinase inhibitor. |
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Authors: | A Yl?nen A Rinne J Herttuainen J Bogwald M J?rvinen N Kalkkinen |
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Institution: | Institute of Biotechnology, Protein Chemistry Laboratory, University ofHelsinki, Finland. aylonen@operoni.helsinki.fi |
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Abstract: | We describe the purification and characterization of two novel cysteine proteinase inhibitors found in Atlantic salmon skin. One of these, salmon kininogen, has a molecular mass of 52 kDa as determined by matrix-assisted laser desorption/ionization time-of-flight MS, is multiply charged with pI values of 4.0, 4.2 and 4.6 and shows homology to kininogens including the bradykinin motif. The other, salarin, has a molecular weight of 43 kDa, a pI of 5.1 and shows weak homology to cysteine proteinases. Both proteins are N- and O-glycosylated and inhibit papain and ficin but not trypsin. |
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