植物抗冻蛋白及抗冻性分子改良

概述了植物抗冻蛋白及其相关基因的研究现状,主要包括植物低温诱导蛋白、具有热滞活性的植物抗冻蛋白及其相关基因的分离、鉴定与表达调控,以及植物抗冻性基因工程研究动态.在此基础上,讨论了该领域研究中的主要问题、发展趋势及近期研究热点.

Antifreeze Proteins and Molecular Genetic Improvement in Freezing Resistance of Plants

Over the past several years, the proteins and genes associated with freezing resistance and molecular genetic improvement in freezing resistance of plants have been widely studied. The recent progress of research made at home and abroad on low-temperature-induced proteins and antifreeze proteins (AFPs) with thermal hysteresis (THA) and the identification and expression regulation of low-temperature-induced genes are reviewed in this paper. Recent advances in the approaches of gene engineering that have been successfully taken in the molecular improvement of plant freezing resistance are also reviewed. Emphasis is placed on the transformation and expression of the freez- ing resistance genes cloned from overwintering insects, polar fishes and plant materials. Finally, some unsolved problems and the trend of research on physiological function and mechanism of AFPs, and the application of modern biotechnology to molecular improvement in freezing resistance of plants are discussed.