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Expression of <Emphasis Type="Italic">cspH</Emphasis> upon nutrient up-shift in <Emphasis Type="Italic">Salmonella enterica</Emphasis> serovar Typhimurium
Authors:Bae?Hoon?Kim  Hyeon?Guk?Kim  Gun?Ick?Bae  Iel?Soo?Bang  Sung?Ho?Bang  Jong?Hak?Choi  Email author" target="_blank">Yong?Keun?ParkEmail author
Institution:(1) School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea;(2) Department of Biology, Hanseo University, Seosan, 356-706, South Korea;(3) Department of Radiologic Technology, College of Health Sciences, Korea University, Seoul, 136-703, South Korea
Abstract:The gene cspH , which encodes one of the cold-shock proteins in Salmonella enterica serovar Typhimurium, has previously been reported to be induced during early exponential phase at 37°C. In the present study, the expression of cspH upon nutrient up-shift at 37°C was investigated and found to be affected by DNA gyrase and DNA-binding protein Fis. When cells at stationary phase were subcultured into a rich medium, the mRNA level of cspH increased dramatically prior to the first cell division. However, when the cells were treated with DNA gyrase inhibitors, cspH mRNA was not induced upon nutrient up-shift. The low level of DNA superhelical density at the cspH promoter in part affected the expression of cspH mRNA in vitro. In addition, a fis-deficient strain had a lower level of cspH mRNA than the wild-type upon nutrient up-shift. Finally, a cspH–lacZ construct, in which the putative binding region for Fis was deleted in the cspH promoter, expressed a low level of LacZ, in contrast to the native cspH–lacZ construct.This revised version was published online in July 2004 with corrections to Fig. 4.
Keywords:cspH  DNA gyrase  Nutrient up-shift  Fis
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