Isolation and preliminary characterization of plasmid in Bacillus licheniformis strain MP3 |
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| Authors: | C. Parini Maria Grazia Fortina Manuela Roggiani P.L. Manachini |
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| Affiliation: | Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Industrial, Milano 20133, Italy |
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| Abstract: | A restriction analysis of a single covalently closed circular plasmid isolated from Bacillus licheniformis strain MP3 was performed. A restriction map of the 6·44 kb plasmid is presented. The existence of genetic markers such as antibiotics and heavy metal ions resistance was investigated. The availability of two single restriction sites and the stability of this plasmid make it ideal for development as a cloning vector. |
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