Effects of Supplementation of Various Medium Components on Chinese Hamster Ovary Cell Cultures Producing Recombinant Antibody |
| |
Authors: | Do Yun Kim Joon Chul Lee Ho Nam Chang Duk Jae Oh |
| |
Institution: | (1) Department of Bioscience and Biotechnology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, Korea;(2) Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, Korea;(3) Korea Biotechnology Commercialization Center, Korea Institute of Industrial Technology, 994 Dongchoon-Dong, Yeonsu-Gu, Incheon, Korea |
| |
Abstract: | Thirteen vitamins, twenty amino acids, hormones, inorganic salts, and other chemical agents, which constitute typical serum-free
media, were evaluated for the development of fortified medium to enhance cell growth and productivity of recombinant antibody
in the cultures of the recombinant Chinese hamster ovary (rCHO) cells. Two different rCHO cell lines, rCHO-A producing recombinant
antibodies against the human platelet and rCHO-B secreting recombinant antibodies against the S surface antigen of Hepatitis
B, respectively, were cultivated in batch suspension mode. Concentration of interested component in the tested medium was
doubled to examine the fortification effect. Growth of rCHO-A cell and its antibody production were slightly improved with
addition of either choline chloride, folic acid, thiamine⋅HCl, or LongTMR3IGF-I. On the other hand, in the cultivation of rCHO-B cell which was more sensitive to its environmental changes, hormones
such as LongTMR3IGF-I and triiodothyronine (T3) as well as various vitamins involving choline chloride, i-inositol, niacinamide, pyridoxine HCl, and thiamine⋅HCl enhanced
the cell growth and antibody production. Particularly, when concentration of consuming amino acid was doubled, remarkable
increase in specific productivity was served, resulting in high final antibody concentration. These results were believed
to provide a fundamental strategy of medium fortification useful for improvement of recombinant antibody production in serum-free
medium.
These authors contributed equally to this work |
| |
Keywords: | Chinese hamster ovary cell Medium fortification Protein- free medium Recombinant antibody production Serum-free medium |
本文献已被 SpringerLink 等数据库收录! |
|