Low copy number plasmid vectors for gene cloning and for monitoring gene expression |
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| Authors: | Hilde De Reuse Eliette Touati Philippe Glaser Antoine Danchin |
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| Affiliation: | Régulation de l'Expression Génétique, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex, France |
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| Abstract: | Abstract Derivatives of an IncW incompatibility plasmid with a low copy number are described which can be used for gene cloning or for analysing gene expression in conditions similar to those found in the host chromosome. Gene expression can be monitored after construction of operon or protein fusions with the lacZYA operon and measurement in Escherichia coli of the β-galactosidase activity. |
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| Keywords: | Plasmid gene cloning gene expression low copy number vector |
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