Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on beta-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH |
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Authors: | M Nagao T Ishibashi T Okayasu Y Imai |
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Affiliation: | Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan |
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Abstract: | The addition of glucose to yeast cells activates proton efflux mediated by the plasma membrane ATPase. Accordingly, the ATPase activity of purified plasma membranes is increased up to 10-fold. The activated ATPase has a more alkaline pH optimum, better affinity for ATP and greater sensitivity to vanadate than the non-activated enzyme. All these changes are reversed by washing the cells free of glucose. This suggests two states of the ATPase which are interconverted by a covalent modification. As glucose does not affect the phosphorylation of plasma membrane polypeptides, other type of covalent modification may be involved. |
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Keywords: | Immunochemical evidence Microsome Fatty acid Chain elongation |
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