不同造模方法建立脓毒症大鼠模型的炎症指标及器官功能障碍指标的比较

摘要 目的：通过建立大肠杆菌感染型、脂多糖诱导型、宿主屏障破坏（盲肠结扎）型脓毒症模型，评估具有代表性的造模方式。方法：取SD大鼠设置为：大肠杆菌组、脂多糖组、盲肠结扎组、对照1组、对照2组、对照3组，每组10只。于造模后12 h、24 h、36 h、48 h内检测炎症指标：白介素-6（IL-6）、降钙素原（PCT），凝血功能指标：凝血酶原时间（PT）、活化部分凝血活酶时间（APTT），器官功能障碍指标：肌酐（Cre）、谷丙转氨酶（ALT）、心肌肌钙T（cTnT）及动脉血气分析指标：动脉血氧分压（PaO₂）、动脉血二氧化碳分压（PaCO₂）水平变化。结果：与对照1组、对照2组、对照3组比较，12 h-48 h内，大肠杆菌组、脂多糖组、盲肠结扎组大鼠IL-6、PCT、PT、APTT、Cre、ALT，cTnT，PaCO₂升高（P<0.05），PaO₂水平降低（P<0.05），且脂多糖组及盲肠结扎组IL-6、PCT、PT、APTT、Cre、ALT，cTnT水平均高于大肠杆菌组（P<0.05），PaO₂水平低于大肠杆菌组（P<0.05）。结论：大肠杆菌造模、静脉注射脂多糖造模及盲肠结扎造模均可复制脓毒症模型，且静脉注射脂多糖及盲肠结扎造模大鼠的病情严重程度高于大肠杆菌造模。

Comparison of Inflammation Indexes and Organ Dysfunction Indexes in Sepsis Rat Models Established by Different Modeling Methods

ABSTRACT Objective: The representative modeling methods were evaluated by establishing the sepsis models of Escherichia coli infection type, lipopolysaccharide induced type and host barrier destruction (cecal ligation). Methods: SD rats were gathered and set up as: Escherichia coli group, lipopolysaccharide group, cecal ligation group, control group 1, control group 2, control group 3, with 10 rats in each group. Inflammatory indexes: interleukin-6 (IL-6), procalcitonin (PCT), coagulation function indexes: prothrombin time (PT), activated partial thromboplastin time (APTT), organ dysfunction indexes: creatinine (Cre), alanine aminotransferase (ALT), myocardial calcium t (cTnT) and arterial blood gas analysis indexes: arterial partial pressure of oxygen (PaO₂), arterial blood partial pressure of carbon dioxide (PaCO₂) level changes were detected within 12 h, 24 h, 36 h, and 48 h after modeling. Results: Compared with control group 1, control group 2, and control group 3, within 12 h-48 h, the IL-6, PCT, PT, APTT, Cre, ALT, cTnT and PaCO₂ increased in Escherichia coli group, lipopolysaccharide group and cecal ligation group (P<0.05), the PaO₂ level decreased (P<0.05), and the levels of IL-6, PCT, PT, APTT, Cre, ALT, and cTnT in the lipopolysaccharide group and the cecal ligation group were higher than those in the Escherichia coli group (P<0.05), the PaO₂ level was lower than that in the Escherichia coli group (P<0.05). Conclusion: All the Escherichia coli modeling, lipopolysaccharide intravenous injection modeling and cecal ligation modeling can replicate the sepsis model, and the severity of the disease in rats established by lipopolysaccharide intravenous injection and cecal ligation is higher than that in rats established by Escherichia coli modeling.