Expression of FLK1 and CD146 at day 7 following reperfused acute myocardial infarction |
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| Authors: | Sheng Kang Yue-jin Yang Yi-ling Wu Qing-zhi Wang Yue Li Yi Tian Yu-tong Cheng |
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| Institution: | 1. Department of Cardiology, Cardiovascular Institute & Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China;2. The Integration of Traditional and Western Medical Research Academy of Hebei Province, Shijiazhuang Hebei Province, China;1. Department of Internal Medicine and Gastroenterology;2. Institute of Anatomy and Cell Biology;3. Institute of General Pathology;4. Institute of Haematology;5. Department of Surgical Sciences, Gemelli University Hospital, Catholic University of Sacred Heart, Rome, Italy;1. Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea;2. Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinery Medicine, Seoul National University, Seoul, Republic of Korea;3. Laboratory of Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea;4. Research Institute for Veterinary science, Seoul National University, Seoul, Republic of Korea;1. Instituto Mexicano del Petróleo, Eje Lázaro Cárdenas 152, 07730 Ciudad de México, Mexico;2. Instituto Politécnico Nacional, UPALM, Col. Zacatenco, 07738 Ciudad de México, Mexico;3. Benemérita Universidad Autónoma de Puebla, Av. San Claudio CU, 72570 Puebla, Mexico;4. Universidad del Istmo, 70760, Sto. Domingo Tehuantepec, Oaxaca, Mexico;1. Stem Cells and Diabetes Section, National Centre for Cell Science, Pune, India;2. Division of Animal Sciences, Agharkar Research Institute, Pune, India;3. Division of Tissue Engineering and Regeneration Technologies, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India;4. Tissue Engineering and Banking Laboratory, National Center for Cell Science, Pune, India |
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| Abstract: | ObjectivesTo investigate the expression of FLK1, CD146 and microvessel density of angiogenesis at the first week of reperfused acute myocardial infarction (AMI).Methods16 of mini-swines (20 to 30 Kg) were randomly assigned to the sham-operated group and the AMI group. Pathologic myocardial tissue was collected at day 7 following reperfusion and detected by dual immunochemistry, real-time quantitative polymerase chain reaction and western blot.ResultsThe infarcted area had higher FLK1 mRNA expression than the sham-operated area and the normal area (all P < 0.05), and the infarcted and marginal areas showed higher CD146 protein expression than the sham-operated area (all P < 0.05), but the microvessel density (CD31 positive expression of microvessels/HP) was not significantly different between the infarcted area and the sham-operated area (8.92 ± 3.05 vs 6.43 ± 1.54)(P > 0.05).ConclusionFLK1 and CD146 expression significantly increase in the infarcted and marginal areas, and the microvessel density of angiogenesis in the infarcted area is similar to normal microvessel density of healthy heart tissue, suggesting that FLK1 and CD146 are possible associated with angiogenesis at day 7 following reperfused acute myocardial infarction. |
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