Characterization and stability studies on surfactant,detergent and oxidant stable α-amylase from marine haloalkaliphilic Saccharopolyspora sp. A9 |
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| Authors: | Samrat Chakraborty Abhijeet Khopade Ren Biao Wang Jian Xiang-Yang Liu Kakasaheb Mahadik Balasaheb Chopade Lixin Zhang Chandrakant Kokare |
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| Institution: | 1. Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth University, Poona College of Pharmacy,Pune 411 038, India;2. Chinese Academy of Science Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China;3. Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India;4. STES, Sinhgad Institute of Pharmacy, Narhe, Pune 411041, India |
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| Abstract: | A halopalkaliphilic marine Saccharopolyspora sp. stain A9 with an ability to produce surfactants, oxidant and detergent stable α-amylase was isolated from marine sediments collected from west coast of India. The α-amylase from strain A9 was purified to homogeneity with the aid of ammonium sulfate precipitation and gel filtration chromatography by using Sephadex G-75, insoluble corn starch and sephacryl S-100 column, with a 39.01-fold increase in specific activity. SDS-PAGE and zymogram activity staining showed a single band equal to molecular mass of 66 kDa. Enzyme was found to be stable in presence of wide range of NaCl concentration with maximum activity found at 11% (w/v) of NaCl. Enzyme showed remarkable stability towards laboratory surfactants, detergents and oxidants. Glucose, maltose and maltotriose were the main end product of starch hydrolysis, indicating it is α-amylase. |
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