Cryopreservation of mononuclear cells before extracorporeal photochemotherapy does not impair their anti-proliferative capabilities |
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Authors: | Etienne Merlin Dalil Hannani Richard Veyrat-Masson Jacques Chassagne Fraçoise Gabert Marc Berger François Deméocq Joël Plumas Justyna Kanold |
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Affiliation: | 1. Service de médecine interne, CHI, Créteil, France;2. Centre de Référence des Microangiopathies Thrombotiques, AP-HP, Paris, France;3. Service d’hématologie biologique, Hôpital Lariboisière, Paris, France;4. Université Paris Diderot, Sorbonne Paris Cité, Paris, France;5. Service de Médecine Interne, Hôpital la Pitié-Salpétrière, Paris, France;6. Service de médecine interne, CHU Charles Nicolle, Rouen, France;7. Service d’hématologie, Hôpital Saint Antoine, Paris, France;8. Inserm U1009, Institut Gustave Roussy, Villejuif, France |
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Abstract: | Background aimsThe clinical benefits of extracorporeal photochemotherapy (ECP) are well recognized, but its clinical use is limited by logistical difficulties, especially because of the need to perform repeated aphereses. The cryopreservation of mononuclear cells could allow maintenance of the ECP schedule while reducing the number of aphereses. The aim of this work was to assess whether previous cryopreservation impairs the immunomodulatory function of ECP-treated peripheral blood mononuclear cells (PBMC).MethodsFresh or previously cryopreserved PBMC were exposed to ECP and added on day 0 into a mixed leukocyte reaction. Proliferation of alloreactive lymphocytes was measured by carboxyfluorescein succinimidyl ester (CFSE) dye dilution. Apoptosis was quantified by annexin–7AAD staining.ResultsECP-induced apoptosis was slightly increased in cryopreserved cells but the kinetics of apoptosis were similar to fresh cells. Lymphocytes stimulated in the presence of ECP-treated PBMC displayed a significant decrease in proliferation. The suppression was enforced when ECP-treated cells had been activated previously by allogeneic stimulation. Cryopreservation before ECP exposure did not impact apoptosis triggering or anti-proliferative properties of ECP-treated cells.ConclusionsCryopreservation before ECP does not impair the immunomodulatory effects of treated cells. These data warrant investigation of the clinical use of cryopreserved PBMC for ECP. |
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