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Preliminary validation of ERBB2 expression regulated by miR-548d-3p and miR-559 |
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| Authors: | Hong Chen Jian guo Sun Xue-wu Cao Jian-ping Xu Zheng-tang Chen |
| Institution: | a Institute of Cancer Research, Xinqiao Hospital, Third Military Medical University, Xinqiao Street No. 2, Shapingba Di, Chongqing 400037, PR China b Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China c Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China d Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China e Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, PR China f Department of Clinical Oncology, Kunming General Hospital of PLA, Kunming, Yunnan 650032, PR China |
| Abstract: | ERBB2 overexpression occurs in numerous types of primary human tumors and alterations in microRNA (miRNA) expression have been associated with tumor suppression or tumorigenesis in human cancer, nevertheless, little is known about natural miRNAs acting on ERBB2. In this study, bioinformatical analysis of the 3′-UTRs of ERBB2 revealed the target elements for miR-548d-3p and miR-559. Moreover, a predicted miRNA/mRNA interaction experimental validation showed that both miR-548d-3p and miR-559 can interact specifically with the 3′-UTR of the ERBB2 mRNA. And miR-548d-3p plus miR-559 transfection showed a cooperative regulation of translationally repressing ERBB2 mRNA rather than by either miR-548d-3p or miR-559 alone. These results not only support the idea that different miRNAs can simultaneously and cooperatively repress a given target mRNA but also preliminarily validate the role of miR-548d-3p and miR-559 in regulating the ERBB2 expression. These data provide molecular basis for the application of miRNAs in ERBB2-targeted therapy. |
| Keywords: | ERBB2 MicroRNA miR-548d-3p miR-559 Target |
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