Identification and characterisation of thirteen new microsatellites for Atlantic cod (<Emphasis Type="Italic">Gadus morhua</Emphasis> L.) from a repeat-enriched library |
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| Authors: | Jon-Ivar Westgaard Tekle Tafese Mette Serine Wesmajervi Jørgen Stenvik Kjersti Turid Fjalestad Børge Damsgård Madjid Delghandi |
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| Institution: | 1.Norwegian College of Fishery Science,University of Troms?,Troms?,Norway;2.Department of Aquaculture,Norwegian Institute of Fisheries and Aquaculture Research,Breivika,Norway |
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| Abstract: | A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population.
The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122–0.907 and
0.673–0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy–Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination
of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing
linkage maps of Atlantic cod.
Jon-Ivar Westgaard and Tekle Tafese have contributed equally to the work. |
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| Keywords: | Atlantic cod Enriched genomic library Microsatellites Type II markers Dinucleotide repeats |
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