Circular dichroism spectra of glutamate dehydrogenase plus reduced nicotinamide adenine dinucleotide. |
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| Authors: | B D Wells |
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| Affiliation: | Department of Chemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306 USA |
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| Abstract: | Circular dichroism (CD) spectra are reported for various concentrations of glutamate dehydrogenase in order to determine any role of protein aggregation on NADH-binding spectra. These CD spectra do appear to be sensitive to enzyme aggregation. These spectra raise some doubt about previous interpretation of CD spectra as direct evidence for a second NADH binding-site. |
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