| 氯化锂对KT-1/A3细胞增殖和凋亡的影响 |
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| 引用本文： | 唐丽.氯化锂对KT-1/A3细胞增殖和凋亡的影响[J].激光生物学报,2003,12(4):264-268. |
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| 作者姓名： | 唐丽 |
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| 作者单位： | 中南大学湘雅医院新生儿科,中国湖南,长沙,410008 |
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| 摘 要： | 目的：研究氯化锂(LiCl)在体外对KT—1／A3白血病细胞增殖及凋亡的影响。方法：采用液体培养实验，MTT实验，集落培养实验为指标观察LiCl对KT—1／A3细胞增殖的影响，采用DNA片段凝胶电泳及流式细胞检测为指标检测细胞凋亡。结果：①不同浓度的LiCl(5mM—25mM)对KT—1／A3细胞具有抑制增殖的作用，这种增殖抑制作用呈剂量依赖关系。②在LiCl(20mM)作用72h的DNA凝胶电泳谱可见DNA Ladder及流式细胞仪检测可见凋亡特有的AP峰，提示LiCl可诱导KT—1／A3细胞凋亡。绪论：LiCl能抑制KT—1／A3白血病细胞增殖和诱导凋亡。
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| 关 键 词： | 氯化锂（LiCl） 白血病 KT—1／A3细胞株 细胞培养 增殖 凋亡 |
| 文章编号： | 1007-7146(2003)04-0264-05 |
| 收稿时间： | 2003/4/15 |
| 修稿时间： | 2003年4月15日 |
Effects of Lithium Chloride on Proliferation and Apoptosis of KT-1/A3 Leukemia Cells |
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| TANG,Li.Effects of Lithium Chloride on Proliferation and Apoptosis of KT-1/A3 Leukemia Cells[J].ACTA Laser Biology Sinica,2003,12(4):264-268. |
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| Authors: | TANG Li |
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| Abstract: | Objective: The effects of lithium chloride on proliferation inhibition and apoptosis induction of human leukemia cell line (KT1/A3) were studied. Methods: The effect of lithium chloride on KT1/A3 cell proliferation was evaluated by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. The effect of lithium chloride on KT1/A3 cell apoptosis was verified by the DNA fragments appeared in agarose gel electrophoresis and the AP apex showed by flow cytometry . Results: The proliferation of KT1/A3 leukemia cells was inhibited by lithium chloride(5mM25mM)in a dosedependent manner. And KT1/A3 leukemia cells were induced toward apoptosis by lithium chloride(20 mM). |
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| Keywords: | lithium chloride leukemia KT-1/A3 cells line cells cultured proliferation apoptosis |
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