A SIMPLIFIED METHOD OF DNA DISTRIBUTION ANALYSIS |
| |
| Authors: | P N Dean |
| |
| Institution: | Biomedical Sciences Division, Lawrence Livermore Laboratory, University of California, Livermore, California, U.S.A. |
| |
| Abstract: | Current methods of analysing DNA distributions utilize complex mathematical expressions that require the use of large non-linear curve fitting methods and, consequently, large computers. This paper presents a new method of analysing DNA distributions of asynchronously growing or mildly perturbed cells. The S phase fraction is obtained by fitting a second degree polynomial to that part of the distribution, mid S phase, which is not influenced by either the G1 or the G2+ M peaks. The method is simple and fast, it exceeds the accuracy of other methods and it can be used on a large desk calculator or mini-computer. |
| |
| Keywords: | |
|
|
