猪白细胞Ⅱ类抗原基因多态性研究进展 |
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作者姓名: | 周波 王林云 谈永松 张似青 |
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作者单位: | 1.南京农业大学动物科技学院,南京 210095;2.上海市农业科学院畜牧兽医研究所,上海 2011061.College of Animal Science,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Animal Husbandry and Veterinary Medicine,Shanghai Academy of Agricultural Science,Shanghai 201106,China |
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基金项目: | 高等学校博士学科点专项科研基金(编号:1999030704)资助项目 |
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摘 要: | 本文对SLAⅡ类基因的染色体图谱定位、分子结构、基因分型及其多态性的研究进展作了介绍。重点介绍了SLAⅡ类基因的RFLP基因分型及其多态性研究。猪的主要组织相容性复合物Ⅱ类抗原基因座位存在不同程度的等位基因多态性。RFLP技术是一种简便而快捷的SLAⅡ类等位基因分型方法。SLAⅡ类基因的多态性和抗原肽结合位点密切相关,这些位点能结合处理过的抗原,然后递呈给T细胞。另外,在编码β1链的SLA-DRB1基因和SLA-DQB基因中有4个富含GC序列区。Abstract:This article gave a detailed introduction about regional map,molecular structure,genotyping and polymorphism of SLA class Ⅱ genes.The pig major histocompatibility complex (MHC) class Ⅱ antigens have been known to exhibit a different degree of allelic polymorphism.The locus-specific oligonucleotide primers and RFLP analysis provide a simple and rapid method for genotyping expressed SLA class Ⅱ from genomic DNA.SLA class Ⅱ polymorphism was related to the antigenic peptide binding sites.Detailed analysis of sequences showed that there were 4 GC-rich sequences in exon 2 of SLA-DQB and SLA-DRB1 genes.
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关 键 词: | RFLP swine 多态性 Key words SLAⅡ类基因 猪 |
文章编号: | 0253-9772(2003)05-0611-04 |
修稿时间: | 2002-08-23 |
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