Dual effect of prolactin on protein kinase C activity in CHO cells expressing functional prolactin receptors |
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Authors: | M C Audy MD P Vacher A M Vacher B Dufy |
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Institution: | (1) Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II, 146, rue Léo-Saignat, BP 22, F-33076 Bordeaux Cedex, France |
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Abstract: | We investigated the effects of prolactin (PRL) on the protein kinase C (PKC) activity in Chinese hamster ovary (CHO-E32) cells stably transfected with rabbit mammary gland PRL receptor cDNA. These cells express a functional long form of PRL-R. A 10-min to 2-hour treatment with 5 nM PRL resulted in the translocation of PKC activity from the cytosol to the membrane. Longer treatment (10–24 h) with the same concentration of PRL decreased the PKC activity in both particulate and cytoplasmic fractions. The PRL effect was dose dependent: maximal action was obtained with 1–10 nM. The PRL-induced activation of PKC was blocked by 20 nM staurosporine, a PKC inhibitor. Two inhibitors of tyrosine kinase, herbimycin A (1.75 µM) and genistein (100 µM), had no effect on PRL-induced activation of PKC. |
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Keywords: | Chinese hamster ovary cells Phorbol myristate acetate Prolactin Protein kinase C Tyrosine kinase |
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