Sol-gel encapsulation of lactate dehydrogenase for optical sensing of L-lactate |
| |
Authors: | Li Chia I Lin Yi Hua Shih Cheng Ling Tsaur Jeng Pyng Chau Lai Kwan |
| |
Affiliation: | Department of Chemistry, National Chung Cheng University, Ming-Hsiung, 621, Chia-Yi, Taiwan, ROC. |
| |
Abstract: | Sol-gel encapsulation of lactate dehydrogenase and its cofactor can be employed as a disposable sensor for L-lactate. The sensor utilized the changes in absorbance or fluorescence from the reduced cofactor nicotinamide adenine dinucleotide (NADH) upon exposure to L-lactate. Although, problems such as diminished enzymatic activity and/or leaching of enzyme from the sol-gel matrix occurred, the sol-gel process is sufficiently mild to permit retention of enzymatic activity. The apparent activity of LDH in the sensor is at least 10% of that of the dissolved enzyme. The sensor has a linear dynamic range over the normal physiological L-lactate level and has a long-term storage stability of at least 3 weeks. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|