Brugia pahangi: uptake and incorporation of adenosine and thymidine. |
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Authors: | S N Chen R E Howells |
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Affiliation: | Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, England, United Kingdom |
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Abstract: | The uptake and incorporation of adenosine and thymidine by infective larvae, 10-day-old juvenile, and adult stages of Brugia pahangi were investigated using scintillation counting and autoradiographic techniques. No evidence of thymidine incorporation by the worm was obtained in this study. Scintillation counting methods demonstrated that 14C-labeled adenosine was incorporated by all three stages of this filarial worm. Autoradiography, performed on worms incubated in [3H] adenosine from 5 min through 2 hr, revealed that following 5–15 min incubation the greatest degree of adenosine incorporation occurred in the hypodermis and somatic cords. Adenosine incorporation into the deeper body tissues, including the gut, increased significantly with longer periods of incubation. The results obtained further support the concept that nutrient uptake in B. pahangi occurs by a transcuticular route. |
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Keywords: | Filaria Nematode parasitic Adenosine incorporation Thymidine Scintillation counting Autoradiography |
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