Vitamin E activates CRABP-II gene expression in cultured human fibroblasts, role of protein kinase C |
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Authors: | Gimeno Amparo Zaragozá Rosa Viña Juan R Miralles Vicente J |
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Affiliation: | Departamento de Bioquímica y Biología Molecular, Facultades de Farmacia y Medicina, Avda. Vicent Andrés Estellés s/n, Universidad de Valencia, Valencia, Spain. |
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Abstract: | The treatment of human fibroblasts with different tocopherols in the presence of retinol caused an increase in cytoplasmic retinoic acid binding protein II (CRABP-II) mRNA and protein. The possibility of an involvement of protein kinase C (PKC) in the response to tocopherols was supported by the results obtained with the PKC-specific inhibitors, calphostin C and bisindolylmaleimide I. The effect of alpha-tocopherol was prevented by okadaic acid, suggesting that a protein phosphatase is responsible for PKC dephosphorylation produced by the presence of tocopherols. The results shown support the hypothesis that phosphorylation/dephosphorylation of RXRalpha via PKC may be involved in the regulation of CRABP-II gene expression. |
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