丹参对冻融入胎儿卵巢组织异种移植早期血管生成的影响

目的：研究冻融人胎儿卵巢组织移植早期血管生成过程中微血管形态和密度的改变以及血管生成相关基因mRNA的表达;探讨丹参注射液对移植物血管生成的影响。方法：冻融胎儿卵巢组织异种移植至裸鼠肾被膜下,按给药不同分为对照组（生理盐水）和丹参组（丹参注射液每只0．09g／d）,分别于移植后48h、7d和28d回收移植物。结果：移植后两组卵巢组织微血管密度均明显增多;对照组移植后7d血管密度达峰,丹参组血管密度在移植后48h即已显著上升,此后两个时间段保持相对平稳。Angiopoietin-2mRNA表达在移植后48h两组均显著升高,丹参组上升幅度大于对照组（P〈0．05）。结论：冻融人胎儿卵巢组织新血管生成开始于异种移植后48h内,移植后7d组织内微血管密度达峰。丹参在移植早期应用可以促进移植后血管生成,其机制可能与它增加了血管生成相关因子Ang-2mRNA的表达有关。

The effect of danshen on the angiogenesis of the frozen-thawed human fetal ovarian tissue after transplantation

Aim： ①To investigate the mRNA expression of the key angiogenic growth factors in the grafts after transplantation. ②To investigate the potential impact of Danshen（Chinese Traditional medicine） administration on grafts angiogenesis. Methods： The frozen-thawed ovarian tissue from aborted fetus were xenografted into the renal capsule of the nude mice, recovered 48 h, 7 d and 28 d after respectively. Either Dansher or saline（as the control） was administered after transplantation. Results： The mRNA levels of VEGF showed a temporary raise in 48 h after transplantation, then decreased in one week, and no significant difference was fund between the control group and Danshen group. Ang-2 was increased in 48 h after transplantation, when Danshen group was significantly higher than the control group（ P 〈 0.05）. The microvessel density significantly increased in all the tissues after transplantation. The control group peaked on day 7 after transplantation, while Danshen group peaked in 48 h and kept correspondingly steady after that. Condusion： Early angiogenesis began within 48 h after transplantation of the thawed human fetal ovarian tissue, and its micmvessel density peaked within the first week after transplantation. Our results also suggested that the use of Danshen injection in conjunction with transplantation could facilitate revascularization of the grafts.