银杏内酯B和低氧预适应对小鼠急性缺氧损伤的保护作用

目的：通过观察缺氧预适应和银杏内酯B预处理对小鼠急性缺氧的影响,了解银杏内酯B的脑保护作用。方法：采用小鼠常压缺氧模型,观察小鼠的行为学并记录各组小鼠的最后死亡时间,脑组织含水量,用RT-PCR、Western blot分别检测各组小鼠皮层组织中RTP801mRNA表达和EPO蛋白表达。结果：银杏内酯B和低氧预适应均能明显延长常压缺氧小鼠的存活时间,降低脑水肿程度,并且银杏内酯组和低氧预适应组RTP801mRNA表达和EPO的表达均明显增加。结论：银杏内酯B与低氧预适应具有相类似的对抗小鼠急性低氧的作用,其脑保护作用与上调RTPS01mRNA和EPO蛋白的表达有关。

The protective effects of ginkgolide B and hypoxic preconditioning against acute hypoxia injury in mice

Aim： To investigate the protective effects of ginkgolide B and hypoxic preconditioning against acute hypoxia injury in mice. Methods： Ordinary pressure acute hypoxia model in mice was adopted to observe the ethology, the duration of the death and the degree of brain edema. Meanwhile the expression of RTPS01 mRNA and erythropoietin（EPO） were measured by RT-PCR and Western blot, respectively. Results： Ginkgolide B and hypoxic preconditioning could both prolong the survival time of hypoxia under ordinary pressure, and significantly decreased the degree of brain edema. Besides ginkgolide B and hypoxic preconditioning could both up-regulate the expression of RTP801mRNA and EPO. Conclusion： Ginkgolide B has the similar effect to hypoxic preconditioning against acute hypoxia. Both of these protective effects may be associated with the up-regulation of the expression of RTP801 mRNA and EPO. KEY WORld：