冷应激致雏鸡肺脏DNA的氧化损伤作用

目的：探讨冷应激对雏鸡肺脏DNA氧化损伤的影响。方法：以健康15日龄雏鸡为试验对象,进行冷应激（12±1℃）处理。检测了肺脏MDA含量以及SOD和GSH-Px活性,并应用KCl-SDS沉淀法和荧光检测法检测肺细胞DNA-蛋白质交联（DPC）系数和DNA-DNA交联（DDC）系数。结果：冷应激时,肺脏MDA含量随应激时间的延长逐渐升高,SOD、GSH-Px活性随应激时间的延长呈现上升趋势,肺细胞DPC和DDC含量也均随应激时间的延长呈升高趋势。结论：揭示冷应激可使肺组织氧化-抗氧化平衡破坏,引起肺组织细胞DNA的氧化损伤。

The effect of cold stress on DNA oxidative damage of lung in chicken

Aim： To explore the influence of cold strss on DNA oxidative damage of lung in chicken. Methods： Took 15-day-old healthy chicks as the experimental object, carried on the cold stress（ 12 ± 1 ℃ ） to process. Detected the change of the MDA content, SOD and GSH-Px activity of the lung, and performed KCl-SDS precipitation method and fluorescence detection method to identify the influence of cold strss on DNA-protein crosslinks（DPC） and DNA-DNA crosslinks（DDC） of lung cell in different time. Results： The results were as follow： with the time lapsing during acute cold stress, MDA content gradually increased, the SOD and GSH-Px activity of the lung increased compared with their control group at each stress time point, and the lung cell DPC and DDC coefficient were all gradually increased with the time lapsing. Condusipm： Cold stress could bring about destruction in the lung tissue oxidation-antioxidaut balance, and causes the oxidation damage of DNA.