A direct technique for the preparation of chromosomes from early equine embryos |
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| Authors: | A Romagnano W A King C L Richer M A Perrone |
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| Institution: | Département d'anatomie, Faculté de médecine, Université de Montréal, Canada. |
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| Abstract: | A technique is described for the preparation of banded chromosomes from early equine embryos cultured for less than 10 h in a medium containing bromodeoxyuridine. In addition to standard Giemsa staining and C-banding, chromosomes thus prepared can also be R-banded by either the RBA or the RB-FPG methods. This technique is rapid, repeatable, and limits cell loss, making it ideal for the preparation of early embryos. |
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