| 以线粒体DNA Cytb确立红喉雉鹑和黄喉雉鹑的分类地位 |
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| 引用本文： | 文陇英,包新康,金园庭,刘廼发.以线粒体DNA Cytb确立红喉雉鹑和黄喉雉鹑的分类地位[J].动物分类学报,2009,34(2). |
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| 作者姓名： | 文陇英 包新康 金园庭 刘廼发 |
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| 作者单位： | 1. 乐山师范学院化学与生命科学学院,四川乐山,614004;兰州大学生命科学学院,兰州,730000
2. 兰州大学生命科学学院,兰州,730000 |
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| 基金项目： | 国家自然科学基金重点项目 |
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| 摘 要： | 采用PCR技术获得红喉雉鹑Tetraophasis obscurus和黄喉雉鹑Tetraophasis szechenyii等物种的828 bp Cytb基因片段,雉鹑属Tetraophasis可变位点为26个,并用MEGA3和K-Estimator 6.1软件进行了序列分析.研究发现红喉雉鹑和黄喉雉鹑之间序列差异为3.0%～3.1%,与其它属种间序列差异相比较,两种雉鹑之间的序列差异达到了种的水平;它们地理分布互相替代;形态差异稳定.地理分布、形态特征和遗传特征均验证了它们应为两个独立的种.依据分子进化速率推测,两种雉鹑分歧时间距今2.0Myr左右,与早更新世冰期发生的时间一致.雉鹑起源于青藏高原,随着青藏高原的抬升,早更新世冰期气候变冷,寒温性针叶林的林线下移,大面积高原草甸出现,雉鹑的祖先种群被隔离进化,而形成了两种雉鹑.
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| 关 键 词： | 红喉雉鹑 黄喉雉鹑 分类地位 |
PHYLOGENETIC OF TETRAOPHASIS OBSCURUS AND TETRAOPHASIS SZECHENYII INFERREND FROM MITOCHONDRIAL CYTOCROME B GENE |
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| WEN Long-Ying,BAO Xin-Kang,JIN Yuan-Ting,LIU Nai-Fa.PHYLOGENETIC OF TETRAOPHASIS OBSCURUS AND TETRAOPHASIS SZECHENYII INFERREND FROM MITOCHONDRIAL CYTOCROME B GENE[J].Acta Zootaxonomica Sinica,2009,34(2). |
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| Authors: | WEN Long-Ying BAO Xin-Kang JIN Yuan-Ting LIU Nai-Fa |
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| Institution: | WEN Long-Ying 1,2,BAO Xin-Kang2,JIN Yuan-Ting2,LIU Nai-Fa 2 1.College of Chemistry & Life Sciences,Leshan Teachers College,Leshan Sichuan 614004,China2.College of Life Science,Lanzhou University,Lanzhou Gansu 730000,China |
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| Abstract: | PCR technique was used to get 828?bp cytb gene from samples of Tetraophasis obscurus,Tetraophasis szechenyii and some other genera of Galliformes.26 variable sites were found in Tetraophasis.Sequences were analyzed by some softwares.We discovered that difference between species of T.obscurus and T.szechenyii was 3.0%-3.1%.Compare with difference between species of other genus that difference between T.obscurus and T.szechenyii was up to the extent of species.Also they could be replaced by each other in geog... |
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| Keywords: | Cytb |
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