The bovine lactoferrin gene (LTF) maps to Chromosome 22 and syntenic group U12 |
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| Authors: | M. Schwerin S. Solinas Toldo A. Eggen R. Brunner H. M. Seyfert R. Fries |
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| Affiliation: | (1) Department of Molecular Biology, Research Institute for Biology of Farm Animals, 18196 Dummerstorf, Germany;(2) Department of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zurich, Switzerland;(3) Laboratoire de Génétique biochimique et de Cytogénétique, INRA, Centre de Recherches de Jouy, 78352 Jouy-en-Josas Cedex, France |
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| Abstract: | Five overlapping lambda EMBL-clones, containing the complete bovine lactoferrin gene (LTF), have been used to map this gene by fluorescence in situ hybridization to bovine Chromosome (Chr) band 22q24. Primers derived from promoter and exon I sequences were applied in polymerase chain reactions (PCRs) to DNA samples of a previously characterized panel of somatic cell hybrid lines, allowing the assignment of the bovine lactoferrin locus to syntenic group U12. These results permit the assignment of syntenic group U12 to bovine Chr 22. |
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