柴胡多糖的结构和抗氧化活性分析

用超声波辅助提取法从柴胡中提取、分离得到多糖SAP3。高效液相色谱法测定其相对分子质量,红外光谱、气相色谱和原子力显微镜对其结构进行初步分析。结果表明:柴胡多糖SAP3是组分均一的多糖,由鼠李糖、木糖、甘露糖、葡萄糖、半乳糖组成,平均相对分子质量为3.3×105。红外光谱分析表明:柴胡多糖具有一般多糖类物质的特征吸收峰,单糖残基以吡喃环的形式存在。原子力显微镜显示柴胡多糖糖链具有分支结构,并缠绕形成环状结构。在0.2~1.2 mg/mL的实验浓度范围内,随着多糖质量浓度的升高,柴胡多糖SAP3对.OH的清除率逐渐增大,最大清除率可达45.2%。

Structural analysis and antioxidation activity of bupleurum polysaccharide

Polysaccharide SAP3 was extracted and isolated from bupleurum by ultrasonic-assisted extraction. The relative molecular weight was determined by high performance liquid chromatography. Its chemical structure was analyzed by infrared spectrometry （FTIR） and atomic force microscopy （AFM）. Experimental results showed that the molecular weight of SAP3 was 3.3 x 105. FT1R showed there are characteristic absorption peaks on polysaccharide SAP3 and its monosaccharide residues existed in the forms of pyran rings. AFM revealed that bupleurum polysaccharide had a multi-branched structure and ring structure in linkages of adjacent monosaccharides. Their scavenging actively increased with the increasing of the concentration from 0.2 to 1.2 mg/mL, the highest inhibition rate was 45.2%.