| 诱导表达p27对HEK293细胞生长代谢的影响 |
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| 引用本文： | 叶玲玲刘红李世崇刘兴茂陈昭烈.诱导表达p27对HEK293细胞生长代谢的影响[J].现代生物医学进展,2011,11(21):4001-4004. |
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| 作者姓名： | 叶玲玲刘红李世崇刘兴茂陈昭烈 |
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| 作者单位： | 军事医学科学院生物工程研究所 北京100071 |
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| 基金项目： | 国家“重大新药创制”科技重大专项(No.2009ZX09503-011) |
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| 摘 要： | 目的:研究诱导表达p27对HEK293细胞生长和代谢的影响。方法:将pTet-on载体和响应于Dox的p27诱导表达载体共转染HEK293细胞,随机挑选单克隆细胞株。以细胞周期分布和活细胞密度为主要观察指标,考察稳定转染的细胞在Dox诱导下的细胞生长;以Qglc、Qlac和Qgln为主要观察指标,考察转染细胞在Dox诱导下的细胞代谢。结果:p27基因的表达使HEK293细胞的增殖速度显著降低,G1期细胞比例显著升高,葡萄糖消耗和乳酸生产减少。结论:诱导表达p27基因是对HEK293细胞进行G1期阻滞的一种有效策略。
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| 关 键 词： | HEK293细胞 细胞周期 代谢 p27 诱导表达 |
Effects of p27 Inducible-Expression on the Growth and Metabolism
of HEK293 cells |
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| YE Ling-ling,LIU Hong,LI Shi-chong,LIU Xing-mao,CHEN Zhao-lie.Effects of p27 Inducible-Expression on the Growth and Metabolism
of HEK293 cells[J].Progress in Modern Biomedicine,2011,11(21):4001-4004. |
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| Authors: | YE Ling-ling LIU Hong LI Shi-chong LIU Xing-mao CHEN Zhao-lie |
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| Institution: | △(Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China) |
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| Abstract: | Objective: To investigate the effect of p27 inducible-expression on the growth and metabolism of HEK293 cells.Methods: The vector pTet-on and Dox-responsive p27-expressing vector were co-transfected into HEK293 cells and individual clones were isolated randomly.Cell growth and metabolism were assessed by cell-cycle distribution and viable cell density,Qglc,Qlac and Qgln as indexes,respectively.Results: The expression of p27 increased percentage of G1 cells,reduced consumption of glucose and production of la... |
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| Keywords: | HEK293 cells Cell cycle Metabolism p27 Inducible-expression |
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