| 一株皱叶南蛇藤内生放线菌的分离、鉴定及活性研究 |
| |
| 引用本文： | 何艳,郭泽经,叶仁元,晏菊芳,田永强. 一株皱叶南蛇藤内生放线菌的分离、鉴定及活性研究[J]. 生物技术, 2011, 21(5). DOI: 10.3969/j.issn.1004-311X.2011.05.130 |
| |
| 作者姓名： | 何艳 郭泽经 叶仁元 晏菊芳 田永强 |
| |
| 作者单位： | 1. 四川大学化学工程学院,四川成都,610065
2. 成都地奥制药集团药物研究所,四川成都,610041 |
| |
| 摘 要： | 目的:分离具有抗肿瘤作用与抗菌作用的内生放线菌,并对其进行分子鉴定和系统发育分析.方法:从皱叶南蛇藤中分离内生放线菌,通过滤纸片法和SRB法对其进行抗菌活性和抗肿瘤活性筛选,然后利用菌株形态特征、培养特征、生理生化特性和16S rRNA基因序列分析对活性菌株进行鉴定结果:从皱叶南蛇藤中分离到10株内生放线菌,其中内生放线菌LCB369具有较好的抗菌活性和抗肿瘤活性,该菌对5种致病菌和肝癌细胞株HepG2均有抑制作用.经分子分类学分析鉴定,该菌与Streptomyces microflavus 在同一个分支上,同源性为99%.结论:皱叶南蛇藤内生菌LCB369具有明显抗菌和抗肿瘤作用,经鉴定为链霉菌Streptomyces microflavus.
|
| 关 键 词： | 皱叶南蛇藤 内生放线菌 抗菌活性 抗肿瘤 鉴定 |
Isolation, Identification and Antagonistic Activity of Endophytic Actinomycetes Strain from Celastrus rugosus Rehd.et Wils |
| |
| HE Yah,GUO Ze-jing,YE Ren-yuan,YAN Ju-fang,TIAN Yong-qiang. Isolation, Identification and Antagonistic Activity of Endophytic Actinomycetes Strain from Celastrus rugosus Rehd.et Wils[J]. Biotechnology, 2011, 21(5). DOI: 10.3969/j.issn.1004-311X.2011.05.130 |
| |
| Authors: | HE Yah GUO Ze-jing YE Ren-yuan YAN Ju-fang TIAN Yong-qiang |
| |
| Affiliation: | HE Yan1,GUO Ze-jing1,YE Ren-yuan1,YAN Ju-fang2,TIAN Yong-qiang1,*(1.School of Chemical Engineering,Sichuan University,Chengdu 610065,2.Institute of Materia Medica,Chengdu Diao Group,Chengdu 610041,China) |
| |
| Abstract: | Objective:To isolate and identify an endophytic actinomycete strain which have antimicrobial and antitumor activity.Method:Endophytic actinomycetes were isolated from Celastrus rugosus Rehd.et Wils.Filter paper method and SRB method was used to detect antimicrobial and antitumor activity of strains respectively.The strains were identified according to the morphological,cultural characteristics,physiological and biochemical properties and phylogenetic analysis.Result:10 endophytic actinomycete strains were i... |
| |
| Keywords: | Celastrus rugosus Rehd.et Wils endogenous actinomycetes antimicrobial antitumor identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
