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| 染色体重组对转基因大麦中gfp基因表达的作用 | |
| 引用本文： | 苏琰,黎华,刘知晓,张小蒙,朱雪,金赫日,陈建民. 染色体重组对转基因大麦中gfp基因表达的作用[J]. 生物技术通报, 2011, 0(11) |
| 作者姓名： | 苏琰 黎华 刘知晓 张小蒙 朱雪 金赫日 陈建民 |
| 作者单位： | 1. 扬州大学生物科学与技术学院,扬州225009;安徽巢湖职业技术学院,巢湖238000 2. 扬州大学生物科学与技术学院,扬州,225009 3. 朝鲜元山农业大学生物技术系,江原道 |
| 基金项目： | 国家自然科学基金项目(30471075); 转基因生物新品种培育重大专项(2009ZX08002-011B) |
| 摘 要： | 利用5种转绿色荧光蛋白基因(gfp)大麦不同株系及野生型大麦为材料,对不同转基因株系间以及转基因株系与野生型植株间进行杂交,分别对不同世代植株的根尖、花粉中gfp基因的表达量进行测定.结果表明,不同转基因株系间的根尖、花粉的gfp基因在表达量上存在差异,同一转基因材料的gfp基因表达存在组织差异；gfp基因在杂交后代中作为一个显性基因以孟德尔方式稳定遗传,不同染色体上的gfp基因重组有利于提高持基因表达. |
| 关 键 词： | 绿色荧光蛋白 转基因遗传 转基因表达 大麦 |
The Effect of Chromosome Recombination on Transgene gfp Expression in Transgenic Barley (Hordeum vulgare L.) |
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| Su Yan,Li Hua,Liu Zhixiao,Zhang Xiaomeng,Zhu Xue,Kim Hyok Il,Chen Jianmin. The Effect of Chromosome Recombination on Transgene gfp Expression in Transgenic Barley (Hordeum vulgare L.)[J]. Biotechnology Bulletin, 2011, 0(11) | |
| Authors: | Su Yan Li Hua Liu Zhixiao Zhang Xiaomeng Zhu Xue Kim Hyok Il Chen Jianmin |
| Affiliation: | Su Yan1,2 Li Hua1 Liu Zhixiao1 Zhang Xiaomeng1 Zhu Xue1 Kim Hyok Il3 Chen Jianmin1(1College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,2Chaohu Vocational and Technical College,Chaohu 238000,3Wonsan Agricultural University,Biotechnology Department,Kangwon Province,Emocratic People's Republic of Korea) |
| Abstract: | Five transgenic barley lines with different expression levels of transgene gfp and a non-transgenic barley(Igri) were used as parents to make every possible combinations of crosses.The gfp gene expressions were examined on root tips and pollen of F1,F2 progenies and their parents.The inheritance of foreign gene in transgenic plants was also studied.The intensity of gfp gene expression on root tips and pollen showed significant differences not only among transgenic barley lines but also among different organ... |
| Keywords: | Green fluorescent protein Transgene inheritance Transgene expression Barley |
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