| 葡萄白藜芦醇合酶基因的克隆及序列分析 |
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| 引用本文： | 李小明,郭丽琼,黄秀琴,林俊芳,白曦.葡萄白藜芦醇合酶基因的克隆及序列分析[J].生物技术,2011,21(5). |
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| 作者姓名： | 李小明 郭丽琼 黄秀琴 林俊芳 白曦 |
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| 作者单位： | 1. 华南农业大学食品学院
2. 华南农业大学食品学院;华南农业大学生物质能研究所,广东广州510640 |
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| 基金项目： | 国家自然科学基金，华南农业大学“211工程”项目 |
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| 摘 要： | 目的:从葡萄中克隆白藜芦醇合酶基因vrs1并对其序列进行生物信息学分析.方法:利用葡萄总RNA为模板,采用RT - PCR技术克隆白藜芦醇合酶基因vrs1并亚克隆进T- Vector.利用生物信息学工具对其核酸和蛋白序列进行分析.结果:测序结果显示其cDNA序列全长为1 257bp,含有一个1 179bp的开放阅读框.生物信息学分析表明葡萄白藜芦醇合酶基因编码392个氨基酸,分子量为42.9kDa,理论等电点为5.97,具有芪合酶家族固有的氨基酸保守结构域,二级结构主要由α-螺旋、无规则卷曲、延伸链和β-转角组成.结论:该基因的克隆、生物信息学分析为进一步研究其功能奠定了基础.
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| 关 键 词： | 葡萄 白藜芦醇合酶 基因克隆 序列分析 |
Cloning and Sequence Analysis of Resveratrol Synthase Gene from Vitis vinifera |
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| LI Xiao-ming,GUO Li-qiong,HUANG Xiu-qin,LIN Jun-fang,BAI Xi.Cloning and Sequence Analysis of Resveratrol Synthase Gene from Vitis vinifera[J].Biotechnology,2011,21(5). |
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| Authors: | LI Xiao-ming GUO Li-qiong HUANG Xiu-qin LIN Jun-fang BAI Xi |
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| Institution: | LI Xiao-ming1,GUO Li-qiong1,2,HUANG Xiu-qin1,LIN Jun-fang1,2 *,BAI Xi1(1.College of Food Science,South China Agriculture University,2.Institute of Biomass Energy,Guangzhou 510640) |
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| Abstract: | Objective: Resveratrol synthase gene vrs1 from Vitis vinifera was cloned and its sequence had been analyzed by bioinformatics.Method:Total RNA from Vitis vinifera was used as the template to clone the cDNA sequence of vrs1 by RT-PCR and the vrs1 fragment was subcloned into T-Vector.Bioinformatics softwares were used to analysis the cDNA and protein sequence.Result: The result of sequencing showed that the full length of cDNA is 1 257bp.The gene vrs1 contains an opening reading frame of 1 179bp,which coded f... |
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| Keywords: | Vitis vinifera cv Kyoho resveratrol synthase gene clone sequence analysis |
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