| 生物法合成辛伐他汀 |
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| 引用本文： | 朱丽平,任宇红,孙常磊,周剑平,魏东芝. 生物法合成辛伐他汀[J]. 生物加工过程, 2011, 9(5): 11-16. DOI: 10.3969/j.issn.1672-3678.2011.05.03 |
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| 作者姓名： | 朱丽平 任宇红 孙常磊 周剑平 魏东芝 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 通过提高E.coli BL21(DE3)/pAW31菌株中的酰基转移酶LovD的表达,并以Monacolin J为底物,催化合成辛伐他汀。考察发酵培养基和发酵条件对酰基转移酶LovD表达的影响;采用SDS-PAGE凝胶电泳法检测酰基转移酶LovD表达情况;并建立酶活测定方法,测定酰基转移酶LovD的实际酶活。通过实验确定酰基转移酶LovD摇瓶发酵的最佳条件:发酵培养基为TB培养基,接种量为4%,诱导初始菌密度为0.7(OD600)I,PTG浓度为0.2 mmol/L,在20℃下诱导20 h。在最佳条件下,酰基转移酶LovD的表达水平为100 mg/L,辛伐他汀的产量为1.2 g/L。
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| 关 键 词： | 酰基转移酶 MonacolinJ 辛伐他汀 |
Biosynthesis of simvastatin |
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| ZHU Liping,REN Yuhong,SUN Changlei,ZHOU Jianping,WEI Dongzhi. Biosynthesis of simvastatin[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(5): 11-16. DOI: 10.3969/j.issn.1672-3678.2011.05.03 |
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| Authors: | ZHU Liping REN Yuhong SUN Changlei ZHOU Jianping WEI Dongzhi |
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| Affiliation: | ZHU Liping,REN Yuhong,SUN Changlei,ZHOU Jianping,WEI Dongzhi(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China) |
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| Abstract: | Simvastatin was synthesized from Monacolin J using acyltransferase LovD.The objective of this study was to investigate the expression of acyltransferase LovD,and optimize its medium and fermenting conditions.The level of expression was visualized by sodium dodecyl sulfate-polyacryl-amide gel electrophoresis.To quantify the active amount of LovD expressed under different growth conditions and in different media,an activity assay was used.The optimum fermentation conditions of acyltransferase LovD were determ... |
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| Keywords: | acyltransferase Monacolin J simvastatin |
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