| 绵羊cdh1基因cDNA全编码区的克隆及序列分析 |
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| 引用本文： | 胡甜园,白音巴图,罗奋华,吴应积.绵羊cdh1基因cDNA全编码区的克隆及序列分析[J].生物技术通报,2011(9). |
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| 作者姓名： | 胡甜园 白音巴图 罗奋华 吴应积 |
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| 作者单位： | 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特,010021 |
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| 基金项目： | 教育部春晖计划资助项目(Z2007-1-01036); 内蒙古自治区自然科学基金项目(2009ZD05) |
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| 摘 要： | 在哺乳动物成体睾丸中,精子发生的过程开始于未分化的A型精原细胞的干细胞群.目前已有报道在小鼠未分化的A型精原细胞中特异性表达钙依赖性跨膜黏着蛋白基因(cdh1),但绵羊的cdh1基因全序列未见报道.为了更好地研究绵羊精原干细胞的特性,根据已报道的其他物种的cdh1基因的cDNA保守区设计引物,从成年蒙古绵羊睾丸中提取总RNA,采用RT-PCR和分子克隆方法克隆了蒙古绵羊cdh1基因cDNA全编码区.DNA序列测定结果与牛的核苷酸序列比对,同源性为96.5％,说明该基因在进化上是高度保守的.这为制备绵羊CDHI的抗体奠定了基础,并且为绵羊精原干细胞的分子水平鉴定提供了研究备件.
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| 关 键 词： | 绵羊 cdh1基因 克隆 序列分析 |
Cloning and Analysis of Sheep cdhl Gene |
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| Hu Tianyuan,Bai Yinbatu,Luo Fenhua,Wu Yingji.Cloning and Analysis of Sheep cdhl Gene[J].Biotechnology Bulletin,2011(9). |
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| Authors: | Hu Tianyuan Bai Yinbatu Luo Fenhua Wu Yingji |
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| Institution: | Hu Tianyuan Bai Yinbatu Luo Fenhua Wu Yingji(The Key Laboratory for Mammalian Reproductive Biology and Biotechnology,Ministry of Education,Inner Mongolia University,Hohhot 010021) |
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| Abstract: | In the mammalian testis,spermatogenesis is initiated from a subset of stem cells belonging to undifferentiated type A spermatogonia.In spite of the biologic significance of undifferentiated type A spermatogonia,little is known about their behavior and properties because of lack of specific cell surface markers for analysis.The cdh1 gene has been reported specifically expressed in undifferentiated type A spermatogonia.However,the DNA sequence of cdh1 gene in Ovis aries have not been reported.In this study,we... |
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| Keywords: | Sheep cdh1 gene Cloning Sequence analysis |
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