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| 利用RNAi抑制PARs防治脑缺血性损伤的实验研究 | |
| 引用本文： | 王莹,祝萍,王昆祥,姜海智,王旭东,姜宏俭,郑永慧.利用RNAi抑制PARs防治脑缺血性损伤的实验研究[J].现代生物医学进展,2011,11(22):4256-4259. |
| 作者姓名： | 王莹 祝萍 王昆祥 姜海智 王旭东 姜宏俭 郑永慧 |
| 作者单位： | 1. 哈尔滨医科大学第一医院 干部病房 150023 2. 哈尔滨红十字中心医院 急诊科 150076 3. 佳木斯大学附属第一医院神经内科 154007 4. 哈尔滨医科大学第一医院 神经内科 5. 哈尔滨医科大学第四医院 神经内科 黑龙江 哈尔滨 150001 |
| 摘 要： | 目的:利用RNA干扰技术,通过重组腺病毒导入,抑制脑组织中PARs基因的表达,观察神经功能缺陷评分、缺血脑组织梗死体积的变化,为缺血性脑血管疾病的基因治疗提供实验依据。方法:雄性Wistar大鼠随机分组,以重组腺病毒介导的无序PARs基因shRNA片段、生理盐水进行干预,干预3天后以线栓法建立Wistar大鼠大脑中动脉永久闭塞模型,各组分别于线栓后24h、72h进行神经功能缺陷评分并断头取脑,应用4%TTC染色测脑梗死体积。结果:1.缺血后24h、72h同一时间点:①实验组分别与对照组、生理盐水组对比,神经功能缺陷评分明显降低(P<0.05),脑梗死体积明显减小(P<0.05);②对照组与生理盐水组对比,神经功能缺陷评分、脑梗死体积无明显差异(P>0.05)。2.各组组内缺血后24h、72h对比,神经功能缺陷评分、脑梗死体积各组均有明显差异(P<0.05)。结论:重组腺病毒介导的RNAi能有效抑制脑组织中PARs基因的表达,缩小脑梗死体积,改善神经功能缺陷评分。 |
| 关 键 词： | RNAi PARs 重组腺病毒 脑缺血 |
Research on Inhibition of PARs Gene Expression by RNAi Attenuates Ischemic Brain Injury |
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| WANG Ying,ZHU Ping,WANG Kun-xiang,JIANG Hai-zhi,WANG Xu-dong,JLANG Hong-quan,ZHENG Yong-hui.Research on Inhibition of PARs Gene Expression by RNAi Attenuates Ischemic Brain Injury[J].Progress in Modern Biomedicine,2011,11(22):4256-4259. | |
| Authors: | WANG Ying ZHU Ping WANG Kun-xiang JIANG Hai-zhi WANG Xu-dong JLANG Hong-quan ZHENG Yong-hui |
| Institution: | WANG Ying1,ZHU Ping2,WANG Kun-xiang3,JIANG Hai-zhi4,WANG Xu-dong4,JIANG Hong-quan4,ZHENG Yong-hui5(1 The 1st clinical hospital of Harbin medical university,VIP department 150023,2 Harbin red-cross center hospital,emergency department 150076,3 The 1st clinical hospital of Jiamusi medical university,neurology department 154007,4 The 1st clinical hospital of Harbin medical university,neurology department,5 The 4th clinical hospital of Harbin medical university,neurology department 150001) |
| Abstract: | Objective: To investigate the neuron-protection effects of brain ischemia on gene expression inhibition of recombinant adeno-associated virus(rAAV)-mediated gene transfer to ischemic brain,and to provide basic evidence for gene therapy on cerebral is-chemia.Method: 84 adult male Wistar rats between 16 and 20 weeks of age and weighing 220 to 280g were randomly divided into three groups: the experimental group(EG),the control group(CG),the physiological sodium group(PSG).The rats were given rAAV-RPARs-shRNA i... |
| Keywords: | RNA interference(RNAi) Protease activated receptors(PARs) Recombinant adeno-associated virus(rAAV) Ischemia |
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