| 利用cDNA - AFLP技术分离棉花抗枯萎病相关基因 |
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| 引用本文： | 张伟,郑丽玲,韩璐,马玲玲,魏延宏,张薇.利用cDNA - AFLP技术分离棉花抗枯萎病相关基因[J].生物技术,2011,21(5). |
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| 作者姓名： | 张伟 郑丽玲 韩璐 马玲玲 魏延宏 张薇 |
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| 作者单位： | 1. 石河子大学农学院,新疆石河子,832000
2. 石河子大学生命科学技术学院,新疆石河子,832000 |
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| 基金项目： | 石河子大学科学技术研究发展计划项目 |
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| 摘 要： | 目的:分离出棉花抗枯萎病基因片段,并Blast分析其差异片段.方法:以抗枯萎病的棉花品种中棉12号为材料,采用苗期水培方法于三叶期进行枯萎病菌诱导处理,以不接菌为对照.用64对选择性扩增引物对诱导处理和对照的cDNA进行AFLP分析.结果:得到25个阳性差异片段,在GenBank中进行同源性序列比对,24个片段在GenBank数据库中发现同源序列,有1个功能未知.结论:作物的抗病机理复杂,涉及物质和能量代谢,信号传导,逆境响应及蛋白功能调控等方面.
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| 关 键 词： | 棉花 枯萎病 cDNA - AFLP 差异表达基因 |
Investigation Based on cDNA -AFLP Approach for Separation Fusarium Wilt Resistance Gene in Cotton |
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| ZHANG Wei,ZHENG Li-ling,HAN Lu,MA Ling-ling,WEI Yan-hong,ZHANG Wei.Investigation Based on cDNA -AFLP Approach for Separation Fusarium Wilt Resistance Gene in Cotton[J].Biotechnology,2011,21(5). |
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| Authors: | ZHANG Wei ZHENG Li-ling HAN Lu MA Ling-ling WEI Yan-hong ZHANG Wei |
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| Institution: | ZHANG Wei1,ZHENG Li-ling2,HAN Lu1,MA Ling-ling1,WEI Yan-hong1,ZHANG Wei1*(1.Shihezi University of Agriculture,Shihezi 832000,China,2.Shihezi University,Institute of Life Science and Technology,China) |
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| Abstract: | Objective:To isolate a gene fragment of cotton resistance to Fusarium Wilt,and Blast analysis of the differential fragments.Method:In this study,resistance to Fusarium Wilt of cotton varieties in cotton No.12 for the material,the use of seedlings in water culture method for Fusarium-induced leaf stage treatment to the control without inoculation.With 64 pairs of selective primers for induction treatment and control of cDNA AFLP analysis.Result:The positive difference between 25 fragments in the GenBank sequ... |
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| Keywords: | cotton Fusarium Wilt cDNA-AFLP differentially expressed genes |
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