| 玉米AGPase基因启动子的克隆及鉴定 |
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| 引用本文： | 王帅,吴颖,苏胜忠,刘宏魁,李世鹏,单晓辉,原亚萍. 玉米AGPase基因启动子的克隆及鉴定[J]. 生物技术通报, 2011, 0(9) |
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| 作者姓名： | 王帅 吴颖 苏胜忠 刘宏魁 李世鹏 单晓辉 原亚萍 |
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| 作者单位： | 吉林大学植物科学学院,长春,130062 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08003-024B) |
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| 摘 要： | 以玉米基因组DNA为模板,通过PCR技术扩增得到了腺苷二磷酸葡萄糖焦磷酸化酶AGPase基因编码区上游1912 bp的启动子(AGPasep)序列.该序列包含TATA-box,CAAT-box等一些高等植物特有的启动子基本核心序列,推断其为一种新的启动子.为了验证该序列是否具有启动子功能,构建了含有此序列的植物表达载体pCAM-AGPasep,通过农杆菌介导法转化玉米愈伤组织进行瞬时表达.GUS染色结果表明,该序列可以驱动GUS基因的表达,具有启动子功能.
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| 关 键 词： | 玉米 AGPase启动子 GUS瞬时表达 |
Cloning and Identification of Maize AGPase Gene Promoter |
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| Wang Shuai,Wu Ying,Su Shengzhong,Liu Hongkui,Li Shipeng,Shan Xiaohui,Yuan Yaping. Cloning and Identification of Maize AGPase Gene Promoter[J]. Biotechnology Bulletin, 2011, 0(9) |
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| Authors: | Wang Shuai Wu Ying Su Shengzhong Liu Hongkui Li Shipeng Shan Xiaohui Yuan Yaping |
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| Affiliation: | Wang Shuai Wu Ying Su Shengzhong Liu Hongkui Li Shipeng Shan Xiaohui Yuan Yaping (Plant Science College of Jilin University,Changchun 130062) |
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| Abstract: | Genetic DNA of maize was used as total template to amplify the 1 912 bp promoter upon adenosine diphosphorate glucose pyrophosphorylase AGPase coding region using PCR in this research and named AGPasep.The higher plants basic cored promoter sequences,such as TATA-box and CAAT-box were found among the AGPasep,which is supposed to be a new promoter.To indentify the promoter function of the sequence,we constructed a plant expression vector(pCAM-AGPasep)that was transformed into the maize callus through Agrobac... |
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| Keywords: | Maize AGPase promoter GUS transient expression |
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