| 泥鳅MnSOD基因的电子克隆及序列分析 |
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| 引用本文： | 陈国强,陈忠翔,王萍.泥鳅MnSOD基因的电子克隆及序列分析[J].生物技术,2011,21(5). |
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| 作者姓名： | 陈国强 陈忠翔 王萍 |
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| 作者单位： | 淮海工学院海洋学院,江苏省海洋生物技术重点实验室,江苏连云港222005 |
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| 基金项目： | 淮海工学院引进人才科研启动基金 |
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| 摘 要： | 目的:电子克隆并分析泥鳅MnSOD基因.方法:通过电子克隆泥鳅MnSOD基因的开放阅读框,并对其氨基酸组成、功能结构域、系统进化、信号肽、二级结构、三级结构等信息进行预测分析.结果:通过电子克隆获得泥鳅MnSOD基因的cDNA.该基因的开放阅读框大小为675bp,编码224个氨基酸残基,推导的蛋白质分子量为25kDa,等电点约为8.41,其编码序列与其他物种相比非常保守,与鲤形目淡水鱼的MnSOD在进化上亲缘关系最近.其N端含转运肽,推测它定位于线粒体中.预测泥鳅MnSOD的二级结构及三级结构含有较多的不规则卷曲和α螺旋,其N端为两个长的反平行螺旋,C端则为含有拧成三股折叠片的α+β结构.结论:该研究为泥鳅进一步的分子生物学及抗氧化研究奠定了基础.
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| 关 键 词： | 泥鳅 MnSOD 电子克隆 序列分析 |
In Silico Cloning and Sequence Analysis of MnSOD Gene from Misgurnus anguillicaudatus |
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| CHEN Guo-qiang,CHEN Zhong-xiang,WANG Ping.In Silico Cloning and Sequence Analysis of MnSOD Gene from Misgurnus anguillicaudatus[J].Biotechnology,2011,21(5). |
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| Authors: | CHEN Guo-qiang CHEN Zhong-xiang WANG Ping |
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| Institution: | CHEN Guo-qiang,CHEN Zhong-xiang,WANG Ping*(College of Marine Science,Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyugang 222005,China) |
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| Abstract: | Objective: The MnSOD gene from Misgurnus anguillicaudatus was cloned by in silico technique.Method: The MnSOD gene was cloned by in silico technique.The sequence was analyzed by bioinformatic tools to predict composition of amino acid,domain,phylogenetic tree,signal peptide,secondary structure,tertiary structure,and so on.Result: The result showed that the cDNA contained an ORF with the longest length of 675bp,and encoding 224 amino acids.The molecular weight and pI of deduced protein is 25kDa and 8.41.The ... |
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| Keywords: | Misgurnus anguillicaudatus manganese superoxide dismutase in silico cloning sequence analysis |
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