| Wistar大鼠Pdia3cDNA的克隆及原核表达 |
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| 引用本文： | 乌吉斯古冷,汤睿智,邢万金. Wistar大鼠Pdia3cDNA的克隆及原核表达[J]. 生物技术通报, 2011, 0(9) |
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| 作者姓名： | 乌吉斯古冷 汤睿智 邢万金 |
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| 作者单位： | 内蒙古大学生命科学学院,呼和浩特,010021 |
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| 基金项目： | 国家自然科学基金项目(30960174); 国家大学生创新基金项目(081012607) |
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| 摘 要： | 哺乳动物的精卵融合是一个多分子参与的复杂过程,目前只鉴定出几种影响精卵融合的蛋白因子,对其分子机制还知之甚少.其中位于精子上的Pdja3具有二硫键异构酶活性,可能催化其他精卵融合相关蛋白的二硫键变化而参与精卵膜融合.克隆了Wistar大鼠的Pdia3 cDNA片段,长度为1 620 bp,其中编码区为1 518 bp.测序结果显示,编码区内有两个碱基与GenBank公布的大鼠Pdia3cDNA不同,分别为第147个密码子GAG(Glu)的第1位碱基和第410个密码子AAG(Lys)的第3位碱基,前者造成氨基酸替换,后者仅为多态性.此外,还在大肠杆菌中成功地表达并纯化了GST-Pdia3融合蛋白,为下一步研究大鼠Pdia3在体外影响精卵融合和与其它蛋白的相互作用奠定了基础.
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| 关 键 词： | Wistar大鼠 Pdia3 cDNA克隆 原核表达 |
Molecular Cloning and Prokaryotic Expression of Witstar Rat Pdia3 |
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| Bao Ujisguleng,Tang Ruizhi,Xing Wanjin. Molecular Cloning and Prokaryotic Expression of Witstar Rat Pdia3[J]. Biotechnology Bulletin, 2011, 0(9) |
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| Authors: | Bao Ujisguleng Tang Ruizhi Xing Wanjin |
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| Affiliation: | Bao Ujisguleng Tang Ruizhi Xing Wanjin(School of Life Sciences,Inner Mongolia University,Hohhot 010021) |
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| Abstract: | The process of sperm-egg fusion in mammals is a complex mechanism involving multiple proteins,however,little is known about the molecular mechanism underlying this event,except for some proteins have been identified to affect the fusion efficacy.The Pdia3,which has been detected on the sperm head,has an activity of thioredoxin,and was speculated to participate in this process by catalyzing the disulfide bonds to induce conformational changes in other fusion related proteins.In the present investigation,we c... |
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| Keywords: | Wistar rat Pdia3 cDNA cloning Prokaryotic expression |
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