| 响应面法优化松茸发酵产多糖的培养条件 |
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| 引用本文： | 郝继伟,邱奉同.响应面法优化松茸发酵产多糖的培养条件[J].生物加工过程,2011,9(4):1-5. |
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| 作者姓名： | 郝继伟 邱奉同 |
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| 作者单位： | 临沂大学生命科学学院,临沂,276005 |
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| 基金项目： | 临沂大学社会立项课题项目 |
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| 摘 要： | 运用响应面法对松茸产多糖的发酵培养条件进行优化研究。首先根据C、N源实验结果,利用Plackett-Bur-man设计,对影响多糖产量的相关因素进行评估,筛选出具有显著效应的3个因素:玉米粉、豆粕和KH2PO4。在此基础上,利用最陡爬坡试验逼近以上3个因素的最大响应区域,采用Box-Behnken设计法对各因素的水平组合进行优化,获得松茸产多糖优化发酵的培养条件:玉米粉质量分数4.54%,豆粕质量分数4.96%,KH2PO4质量分数0.15%,MgSO4.7H2O质量分数0.05%,VB1质量分数0.001%,初始pH5.5,摇床转速180 r/min,发酵时间10 d。在此优化培养条件下松茸总多糖产量可达5.97 g/L。
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| 关 键 词： | 实验设计 发酵 多糖 松茸 |
Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology |
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| HAO Jiwei,QIU Fengtong.Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology[J].Chinese Journal of Bioprocess Engineering,2011,9(4):1-5. |
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| Authors: | HAO Jiwei QIU Fengtong |
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| Institution: | HAO Jiwei,QIU Fengtong(College of Life Science,Linyi University,Linyi 276005,China) |
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| Abstract: | Response surface methodology was adopted to optimize fermentation conditions for polysaccharide of Tricholoma matsutake.Firstly,based on the results of carbon sources and nitrogen source experiments,a Plackett-Burman design was used to investigate the effects of different factors on production of polysaccharide,and three significant factors were corn flour,soybean meal,and KH2PO4.The path of steepest ascent was used to define optimal response region for these three factors.Optimal level combinations of fact... |
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| Keywords: | experimental design fermentation polysaccharides Tricholoma matsutake |
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