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| 蜜蜂TPI基因克隆与生物信息学预测 | |
| 引用本文： | 王琦,李玮妮,王荣. 蜜蜂TPI基因克隆与生物信息学预测[J]. 生物技术通报, 2011, 0(9) |
| 作者姓名： | 王琦 李玮妮 王荣 |
| 作者单位： | 1. 长治学院生物科学与技术系,长治,046011 2. 福建农林大学生命科学学院,福州,350002 3. 长治卫生学校,长治,046000 |
| 基金项目： | 长治学院校级课题(2010111) |
| 摘 要： | 利用电子克隆方法获得蜜蜂(Apis mellifera)磷酸甘油醛异构酶(triosephosphate isomerase,TPI)基因,并采用生物信息学方法对该基因编码蛋白从等电点、疏水性/亲水性、二级结构等进行了预测,以及试验验证,结果表明蜜蜂TPI基因全长为1 768 bp,具有完整的开放阅读框架(ORF),并得到了试验证实. |
| 关 键 词： | 蜜蜂 电子克隆 磷酸甘油醛异构酶 生物信息学 |
cDNA Cloning and Bioinformatic Prediction of TPI Gene from Apis mellifera |
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| Wang Qi,Li Weini,Wang Rong. cDNA Cloning and Bioinformatic Prediction of TPI Gene from Apis mellifera[J]. Biotechnology Bulletin, 2011, 0(9) | |
| Authors: | Wang Qi Li Weini Wang Rong |
| Affiliation: | Wang Qi1 Li Weini2 Wang Rong3(1Department of Biology Science & Technology,Changzhi University,Changzhi 046011,2School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,3Changzhi Municipal Health School,Changzhi 046000) |
| Abstract: | Triosephosphate isomerase gene from Apis mellifera cloned by electronic cloning based on the EST sequences from Unigene of NCBI.Some characters of the TPI that can encode amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects,including hydrophobicity or hydrophilicity,pI,secondary structure.Results showed that the full length of TPI gene from Apis mellifera was 1 768 bp and contained a complete ORF which encoded 247 amino acids and the experiment is verified. |
| Keywords: | Apis mellifera Electronic cloning Triosephosphate isomerase(TPI) Bioinformatics |
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