流式细胞术BrdU/DNA 双染法测定云芝糖肽 诱导的Molt-4 细胞周期阻滞

目的:探究云芝糖肽(PSP)对人急性淋巴母细胞白血病Molt-4细胞周期的影响。方法:采用流式细胞术BrdU/DNA双染法获得各时相细胞分布状况和细胞周期的动力学参数。结果:0.1 mg/mlPSP处理12 h后,G2/M期细胞百分比由对照组的11.09%减少至3.69%。DNA合成时间由12.10 h延长至108.40 h。24 h处理组中,S期细胞百分比由对照组的43.29%增加至67.26%,而G0/G1期和G2/M期细胞百分比均减少,G0/G1期细胞百分比由对照组的37.47%减少至27.43%,G2/M期细胞百分比由对照组的19.24%降低至5.31%。DNA合成时间更是由11.95 h延长至114.52 h。结论:PSP对人急性淋巴母细胞白血病Molt-4细胞周期的阻滞作用在于S期,该作用与DNA合成抑制有关。

Polysaccharopeptides Derived from Trametes Versicolor Induce Cell Cycle Retardarce on Molt-4 Cells Determined by Flow Cytometry BrdU/DNA Bivariate Immunostaining Analysis

Objective:To investigate the cell cycle inhibition effects of Polysaccharopeptides derived from Trametes versicolor(PSP) on human acute leukemia Molt-4.Methods:Flow cytometry BrdU/DNA bivariate immunostaining method was used to detect the distribution of each phase and cell cycle kinetic parameters.Results:After treatment by 0.1 mg/ml PSP for 12 h,the percentage of G2/M phase cells were dropped from 11.09%(control) to 3.69%.DNA synthesis time extended from 12.10 h to 108.40 h.In 24 h treatment group,the per...