| 广西巴马小型猪血清淀粉样P物质基因真核载体构建及细胞转染 |
| |
| 引用本文： | 杨柳,潘斌,蒋钦杨,覃庆飞,梁家充,兰干球,蒋和生,郭亚芬. 广西巴马小型猪血清淀粉样P物质基因真核载体构建及细胞转染[J]. 生物技术通报, 2011, 0(11) |
| |
| 作者姓名： | 杨柳 潘斌 蒋钦杨 覃庆飞 梁家充 兰干球 蒋和生 郭亚芬 |
| |
| 作者单位： | 广西大学动物科学技术学院,南宁,530004 |
| |
| 基金项目： | 广西科技基础条件平台建设项目(09-090-09) |
| |
| 摘 要： | 血清淀粉样P物质(Serum Amyloid Pcomponent,SAP)是一种在进化上高度保守的血清糖蛋白,它可与各种类型的原纤维结合,在免疫应答和炎症反应等多种免疫疾病中发挥作用.以广西巴马小型猪肝组织总RNA为模板,利用RT-PCR技术扩增出相应cDNA片段,连接到克隆载体pMD18-T上进行检测,测序结果为675 bp,与GenBank所提供相关序列同源性为100％,并成功构建pEGFP-N1 -SAP重组真核表达载体,利用脂质体(Lipofectamine 2000)介导法将重组质粒导入到NIH-3T3细胞中培养,经转染24 h后,置于倒置荧光显微镜下观察,发现含有重组质粒的NIH-3T3细胞中表达出绿色荧光,为进一步研究SAP基因的功能特点及在试验动物相关疾病模型的应用提供务件.
|
| 关 键 词： | 巴马小型猪 血清淀粉样P物质 pEGFP-N1 细胞转染 |
Eukaryotic Vector Construction and Cell Transfection in Bama Mini-pig Serum Amyloid P Component Gene |
| |
| Yang Liu,Pan Bin,Jiang Qinyang,Qin Qingfei,Liang Jiachong,Lan Ganqiu,Jiang Hesheng,Guo Yafen. Eukaryotic Vector Construction and Cell Transfection in Bama Mini-pig Serum Amyloid P Component Gene[J]. Biotechnology Bulletin, 2011, 0(11) |
| |
| Authors: | Yang Liu Pan Bin Jiang Qinyang Qin Qingfei Liang Jiachong Lan Ganqiu Jiang Hesheng Guo Yafen |
| |
| Affiliation: | Yang Liu Pan Bin Jiang Qinyang Qin Qingfei Liang Jiachong Lan Ganqiu Jiang Hesheng Guo Yafen(College of Animal Science and Technology,Guangxi University,Nanning 530004) |
| |
| Abstract: | Serum Amyloid Pcomponent(SAP) is a kind of seroglycoid which has conserved translation in evolution.SAP link with various kinds of fibril in order to play effect on immune response,inflammatory reaction and related immune diseases.In our test,RT-PCR was performed to gene amplification,that cDNA fragment was using the total RNA of Bama Mini-pig liver tissue as a template.The amplified fragment connected to pMD18-T vector and checked.The sequence result showed 675 bp.The homology was 100% compared with the se... |
| |
| Keywords: | Bama Mini-pig SAP pEGFP-N1 Cell transfection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
