| 嗜水气单胞菌毒力基因在传代过程中的稳定性研究 |
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| 引用本文： | 付乔芳,邱军强,胡鲲,杨先乐,安健. 嗜水气单胞菌毒力基因在传代过程中的稳定性研究[J]. 生物技术通报, 2011, 0(9) |
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| 作者姓名： | 付乔芳 邱军强 胡鲲 杨先乐 安健 |
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| 作者单位： | 上海海洋大学术产与生命学院国家水生动物病原库,上海,201306 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(NYCYTX-49-17); 公益性行业(农业)科研专项经费(200803013) |
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| 摘 要： | 为了解嗜水气单胞菌毒力基因在传代过程中的稳定性,对传代前后嗜水气单胞菌(Aeromonas hydrophila,A.hydrophila)的毒力和毒力基因进行测定和检测.采用急性毒性试验方法测定10株嗜水气单胞菌原代、第10代及第20代的菌株对异育银鲫(Carassais auratus gibebio)的半数致死量,通过聚合酶链式反应(PCR)检测原代、第10代及第20代的菌株中5种毒力基因aerA、hlyA、ahpA、altA及ast的变化情况.试验结果表明,嗜水气单胞菌在传代过程中毒力基因分布相对较稳定.但是ahpA基因极易发生变异,在培养基上连续多次传代后,毒力逐渐减弱甚至消失.可推知,ahpA基因与毒力相关性最大,并易受传代影响.
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| 关 键 词： | 嗜水气单胞菌 传代 毒力基因 稳定性 |
Stability of Virulence Genes of Aeromonas hydrophila Strains During Subculture |
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| Fu Qiaofang,Qiu Junqiang,Hu Kun,Yang Xianle,An Jian. Stability of Virulence Genes of Aeromonas hydrophila Strains During Subculture[J]. Biotechnology Bulletin, 2011, 0(9) |
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| Authors: | Fu Qiaofang Qiu Junqiang Hu Kun Yang Xianle An Jian |
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| Affiliation: | Fu Qiaofang Qiu Junqiang Hu Kun Yang Xianle An Jian(College of Fisheries and Life Science,Shanghai Ocean University,State Collection Center of Aquatic Pathogen,Shanghai 201306) |
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| Abstract: | To study the stability of virulence genes of A.hydrophila strains during subculture,10 isolated A.hydrophila strains were subcultured to 20 times on LB agar.Then we observed the change of LD50 and distribution of five virulence factors(aerA,hlyA,ahpA,altA,ast)of A.hydrophila,and the virulence genes were amplified respectively by PCR from template DNA of the 10 strains and their corresponding 20 times subcultured strains.The results showed that the LD50 of Carassais auratus gibebio weakened even losed result... |
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| Keywords: | A.hydrophila Subculture Virulence genes Stability |
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