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荧光蛋白报告基因在植物寄生真菌研究中的应用
引用本文:高必达,钟杰,赵慧茹.荧光蛋白报告基因在植物寄生真菌研究中的应用[J].生物技术通报,2011(9).
作者姓名:高必达  钟杰  赵慧茹
作者单位:湖南农业大学植物病虫害生物学与防探湖南省重点实验室,长沙,410128
基金项目:国家公益性行业(农业)科研专项(200903039)
摘    要:综述菌根真菌、植物内生菌和植物病原真菌等植物寄生真菌转荧光蛋白基因研究现状.介绍转化载体的构建、转化方法及特基因的检测方法,以及转荧光蛋白基因技术在植物寄生真菌侵染过程研究中的应用,指出真菌转荧光蛋白基因存在的问题和展望.

关 键 词:真菌  荧光蛋白  转基因

Application of Fluorescence Protein Reporter Gene in Research of Plant Parasitic Fungi
Gao Bida,Zhong Jie,Zhai Huiru.Application of Fluorescence Protein Reporter Gene in Research of Plant Parasitic Fungi[J].Biotechnology Bulletin,2011(9).
Authors:Gao Bida  Zhong Jie  Zhai Huiru
Institution:Gao Bida Zhong Jie Zhao Huiru(Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Hunan Agricultural University,Changsha 410128)
Abstract:This article were reviewed progress in the transformation of plant parasitic fungi,such as mycorrhizal fungi,endophytic fungi and plant pathogenic fungi with fluorescent protein genes,with emphasis on the construction of vectors,methods of transformation,detection of transgenes,and the application of the fluorescent protein markers in the study of infection process of the plant pathogenic fungi.Finally the existing problems and future interests were also discussed.
Keywords:Fungus Fluorescence protein Transgenic  
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