| 氮源对重组大肠杆菌发酵产L-精氨酸的影响 |
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| 引用本文： | 陈可泉,马江锋,姜岷,韦萍,孙娜亚. 氮源对重组大肠杆菌发酵产L-精氨酸的影响[J]. 生物加工过程, 2011, 9(6): 11-14. DOI: 10.3969/j.issn.1672-3678.2011.06.003 |
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| 作者姓名： | 陈可泉 马江锋 姜岷 韦萍 孙娜亚 |
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| 作者单位： | 南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京,210009 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，国家自然科学基金资助项目，江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 考察有机氮源种类、蛋白胨用量以及(NH4)2SO4用量对重组E.coli发酵产L-精氨酸的影响.结果表明:以蛋白胨作为有机氮源且用量在10 g/L,( NH4 )2SO4用量在15 g/L时,摇瓶发酵产L-精氨酸产量最高,达到9.4g/L.在5L发酵罐进行补料分批培养,通过补加(NH4)2 SO4,L-精氨酸产量可以达到18.8 g/L,比未补加提高了108.9％.
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| 关 键 词： | L-精氨酸 发酵 氮源 重组E.coli |
Effect of nitrogen sources on L-arginine production by recombinant E.coil |
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| CHEN Kequan,MA Jiangfeng,JIANG Min,WEI Ping,SUN Naya. Effect of nitrogen sources on L-arginine production by recombinant E.coil[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(6): 11-14. DOI: 10.3969/j.issn.1672-3678.2011.06.003 |
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| Authors: | CHEN Kequan MA Jiangfeng JIANG Min WEI Ping SUN Naya |
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| Affiliation: | CHEN Kequan,MA Jiangfeng,JIANG Min,WEI Ping,SUN Naya(State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnologyand Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | The effect of nitrogen sources on L-arginine production by recombinant E.coli was studied.The effects of the varieties of organic nitrogen sources,peptone concentration,and(NH4)2SO4 concentration on L-arginine production were investigated.When the concentration of peptone and(NH4)2SO4 were 10 g/L and 15 g/L,respectively,9.4 g/L of L-arginine was obtained in shake flask culture.In batch fermentation with a 5-L fermentor,18.8 g/L of L-arginine was achieved by feeding(NH4)2SO4,thus it was 108.9% higher than th... |
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| Keywords: | L-arginine fermentation nitorgen source recombinant E.coli |
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