| 美洲棉铃虫细胞中dsRNA介导的egfp基因沉默分析 |
| |
| 引用本文： | 赵淑玲,梁昌镛,李敏,王海花,张高瞻.美洲棉铃虫细胞中dsRNA介导的egfp基因沉默分析[J].生物技术通报,2011(11). |
| |
| 作者姓名： | 赵淑玲 梁昌镛 李敏 王海花 张高瞻 |
| |
| 作者单位： | 扬州大学生物科学与技术学院,扬州,225009 |
| |
| 基金项目： | 国家自然科学基金项目(30970138) |
| |
| 摘 要： | 为了分析在美洲棉铃虫细胞( HzAM1)内RNAi的效果,将egfp基因克隆到含有双向T7启动子/终止子的质粒载体中,在体外合成全长的增强型绿色荧光蛋白(egfp)基因dsRNA,将dsRNA和含有能在昆虫细胞内表达eGFP的质粒一起转染HzAM1细胞,分析dsRNA对eGFP表达的抑制作用.结果显示,由egfp基因转录的长dsRNA能有效抑制HzAM1细胞内eGFP的表达,而且该抑制作用表现为剂量依赖效应.但是抑制作用并不彻底,在高剂量的dsRNA处理下,仍有部分细胞内能观察到eGFP的表达.
|
| 关 键 词： | RNA干扰 美洲棉铃虫细胞 体外转录 绿色荧光蛋白 昆虫 |
Analysis of egfp Gene Silencing Mediated with dsRNA in Helicoverpa zea Cells |
| |
| Zhao Shuling,Liang Changyong,Li Min,Wang Haihua,Zhang Gaozhan.Analysis of egfp Gene Silencing Mediated with dsRNA in Helicoverpa zea Cells[J].Biotechnology Bulletin,2011(11). |
| |
| Authors: | Zhao Shuling Liang Changyong Li Min Wang Haihua Zhang Gaozhan |
| |
| Institution: | Zhao Shuling Liang Changyong Li Min Wang Haihua Zhang Gaozhan(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009) |
| |
| Abstract: | Double-stranded RNA-mediated interference(RNAi) has recently emerged as a powerful reverse genetics tool to silence gene expression in multiple organisms,including plants,nematodes and insects.In this study,DNA vector capable of promoting the synthesis of full-length dsRNA of egfp gene in vitro have been constructed,in which egfp gene was inserted into the bidirectional T7 promoter-terminator region.The synthesized dsRNA and a plasmid containing egfp cassette were transfected into HzAM1 cells to analyze eGF... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
