| 表观遗传学研究方法进展 |
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| 引用本文： | 郑小国,陈亮,楼巧君,罗利军.表观遗传学研究方法进展[J].生物技术通报,2011(9). |
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| 作者姓名： | 郑小国 陈亮 楼巧君 罗利军 |
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| 作者单位： | 1. 上海市农业生物基因中心,上海201106;华中农业大学,武汉430070
2. 上海市农业生物基因中心,上海,201106 |
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| 摘 要： | 表观遗传调控是基因表达调控的重要组成部分,已成为当前研究的热点.目前其研究主要集中在DNA甲基化和组蛋白修饰.针对这两种表观修饰,其研究方法也取得了较太进展,一方面方法的是敏度和特异性都在不断提高；另一方面表现修饰的检测正在逐步从定性检测向定量分析方向发展,从个别位点向高通量检测发展.此外,新一代测序技术的应用特大大推动表观遗传研究的发展,包括单分子实时测序法、单分子纳米孔科序法等.综述目前常用的DNA甲基化、组蛋白修饰研究方法以及最新的单分子测序技术,并对它们在表观遗传修饰检测中的应用作了简要对比分析.
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| 关 键 词： | 表观遗传学 DNA甲基化 组蛋白修饰 限制性内切酶酶切法 重亚硫酸盐法 染色质免疫共沉淀 单分子实时测序 单分子纳米孔测序 |
Research Method Progress of Epigenetics |
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| Zheng Xiaoguo,Chen Liang,Lou Qiaojun,Luo Lijun.Research Method Progress of Epigenetics[J].Biotechnology Bulletin,2011(9). |
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| Authors: | Zheng Xiaoguo Chen Liang Lou Qiaojun Luo Lijun |
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| Institution: | Zheng Xiaoguo1.2 Chen Liang 1 Lou Qiaojun1 Luo Lijun1.2(1Shanghai Agrobiological Gene Center,Shanghai 201106,2Huazhong Agricultural University,Wuhan 430070) |
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| Abstract: | Epigenetic regulation has become a hotspot in the study on gene expression regulation.Epigenetic regulation mainly focuses on DNA methylation and histone modification.A great deal of techniques has been developed to detect these epigenetic modifications and great progresses have also been achieved in terms of detection sensitivity and specificity,qualitative and quantitative assay.The next-generation sequencing technologies will greatly promote the development of epigenetic research providing a chance for r... |
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| Keywords: | Epigenetic DNA methylation Histone modification Restriction endonuclease enzyme digestion Bisulfate Chromatin immunoprecipitation(ChIP) Single-molecular real-time sequencing Single-molecule nanopore DNA sequencing |
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